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Analysis of the continuous phase of the modified waxy maize starch suspension

Desse, M., Ang, S., Morris, Gordon, Abu-Hardan, M., Wolf, B., Hill, S. E., Harding, S. E., Budtova, T. and Mitchell, J. R. (2009) Analysis of the continuous phase of the modified waxy maize starch suspension. Carbohydrate Polymers, 77 (2). pp. 320-325. ISSN 0144-8617

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The continuous phase of the suspension of swollen-in-water modified waxy maize starch was analysed. The composition, concentration and molecular weight of the substance released from modified starch granules were determined. Starch granules were swollen in excess water at 73 °C and held at this temperature for 1 min. Centrifugation was used to separate the granules from the supernatant; the latter was then submitted to physico-chemical analysis. Surface tension measurements showed that the supernatant was different from pure water indicating the presence of dissolved polymeric material(s). Differential Scanning Calorimetry and iodine staining results revealed the presence of amylopectin. Analytical Ultracentrifugation and Size Exclusion Chromatography coupled with a Multi-angle Laser Light Scattering were used to determine the sedimentation coefficient and weight-average molecular weight of the soluble amylopectin fraction as well as giving an indication of solution concentration. The molecular weight of dissolved amylopectin was around 1.5 × 106 g/mol and its concentration in the supernatant varied from 0.6 to 6.7 mg/mL for initial 10 mg/mL and 50 mg/mL starch suspensions, respectively. The sedimentation coefficient, weight-average molecular weight and amylopectin concentration in the supernatant all increased non-linearly with the initial starch concentration in the suspension. © 2009 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Amylopectin Analytical ultracentrifugation Continuous phase DSC Iodine staining Physically modified waxy maize starch Size exclusion chromatography-multi-angle laser light scattering Surface tension Capillarity Centrifugation Chromatographic analysis Concentration (process) Differential scanning calorimetry Dissolution Gel permeation chromatography Granulation Industrial chemicals Iodine Lasers Light scattering Molecular weight Refraction Scattering Starch Surface analysis Surface chemistry Surface properties Surface waves Surfaces Suspensions (fluids) Weighing Wetting Size exclusion chromatography Zea mays
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
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Language of Original Document: English
Correspondence Address: Budtova, T.; Mines ParisTech, Centre de Mise en Forme des Matèriaux - (CEMEF), 1Member of the European Polysaccharide Network of Excellence (EPNOE), BP 207, 06904 Sophia-Antipolis, France; email:
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Depositing User: Gordon Morris
Date Deposited: 20 May 2011 10:27
Last Modified: 01 Jul 2011 15:26

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