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Immunological and structural properties of a pectic polymer from Glinus oppositifolius

Inngjerdingen, K. T., Patel, T. R., Chen, X., Kenne, L., Allen, S., Morris, Gordon, Harding, S. E., Matsumoto, T., Diallo, D., Yamada, H., Michaelsen, T. E., Inngjerdingen, M. and Paulsen, B. S. (2007) Immunological and structural properties of a pectic polymer from Glinus oppositifolius. Glycobiology, 17 (12). pp. 1299-1310. ISSN 0959-6658

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Abstract

The aim of this paper was to further elucidate the structure and the immunomodulating properties of the pectic polymer GOA2, previously isolated from Glinus oppositifolius. Enzymatic treatment of GOA2 by endo -α-d-(1→4)-polygalacturonase led to the isolation of three pectic subunits, GOA2-I, GOA2-II, and GOA2-III, in addition to oligogalacturonides. GOA2-I was shown to consist of 1,2-linked Rha p and 1,4-linked Gal p A in an approximately 1:1 ratio, and NMR-analysis showed that the monomers were linked together in a strictly alternating manner. The galactose units in GOA2-I were found as terminal-, 1,3-, 1,6-, 1,4-, 1,3,4-, and 1,3,6-linked residues, while the arabinofuranosyl existed mainly as terminal- and 1,5-linked units. A rhamnogalacturonan-I type structure was suggested being the predominant part of GOA2-I. According to linkage analysis GOA2-II and GOA2-III contained glycosidic linkages characteristic for rhamnogalacturonan-II type structures. GOA2 was shown by sedimentation velocity in the analytical ultracentrifuge, to have a broad degree of polydispersity with a mode s20,w value of ∼1.9 S, results reinforced by atomic force microscopy measurements. The polydispersity, as manifested by the proportion of material with s20,w > 3 S, decreased significantly with enzyme treatment. The abilities of GOA2, GOA2-I, GOA2-II, and GOA2-III to induce the proliferation of B cells, and to exhibit complement fixing activities were tested. In both test systems, GOA2-I showed significantly greater effects compared to its native pectin GOA2. GOA2-I was in addition shown to exhibit a more potent intestinal immune stimulating activity compared to GOA2. The ability of GOA2 to induce secretion of proinflammatory cytokines was examined. Marked upregulations in mRNA for IL-1βB2; from rat macrophages and IFN-γ from NK cells were found. © The Author 2007. Published by Oxford University Press. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Glinus oppositifolius Immunomodulation Pectic polymer Rhamnogalacturonan gamma interferon Glinus oppositifolius extract immunomodulating agent interleukin 1beta messenger RNA pectic polymer GOA2 pectic polymer GOA2 I pectic polymer GOA2 II pectic polymer GOA2 III plant medicinal product polymer polysaccharide unclassified drug Aizoaceae animal cell animal tissue article atomic force microscopy complement fixation test controlled study cytokine release drug isolation drug structure immunostimulation lymphocyte proliferation macrophage mouse natural killer cell nonhuman priority journal rat structure analysis upregulation Animals Cell Proliferation Galactose Humans Inflammation Killer Cells, Natural Macrophages Mice Molluginaceae Monosaccharides Pectins Plant Extracts Polymers Rats Rattus
Subjects: R Medicine > RM Therapeutics. Pharmacology
Schools: School of Applied Sciences
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Language of Original Document: English
Correspondence Address: Inngjerdingen, K.T.; Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, Oslo 0316, Norway; email: k.t.inngjerdingen@farmasi.uio.no
Chemicals/CAS: gamma interferon, 82115-62-6; Galactose, 26566-61-0; Monosaccharides; Pectins; Plant Extracts; Polymers; polygalacturonic acid, 9046-38-2; rhamnogalacturonan I; rhamnogalacturonan II
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Depositing User: Gordon Morris
Date Deposited: 13 May 2011 12:56
Last Modified: 01 Jul 2011 16:47
URI: http://eprints.hud.ac.uk/id/eprint/10443

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