Search:
Computing and Library Services - delivering an inspiring information environment

Further observations on the size, shape, and hydration of casein micelles from novel analytical ultracentrifuge and capillary viscometry approaches

Morris, Gordon, Foster, T. J. and Harding, S. E. (2000) Further observations on the size, shape, and hydration of casein micelles from novel analytical ultracentrifuge and capillary viscometry approaches. Biomacromolecules, 1 (4). pp. 764-767. ISSN 1525-7797

Metadata only available from this repository.

Abstract

The size, shape, and hydration of casein micelles were estimated using a combination of sedimentation velocity (time-derivative analysis) in the analytical ultracentrifuge and capillary viscometry applied to skimmed milk. On the basis of sedimentation time-derivative and Wales-van Holde analyses the casein micelles appear as large spherical molecules of s0T,b = 845S, Mw ~ 2.8 x 108, hydrodynamic radius ∼77.8 nm, and ks/[eta] = 1.6. The molecular hydration (i.e., the extent of chemically bound and physically entrained solvent) was calculated to be 3.4 g/g. These results appear to be in good agreement with comparable results from electron microscopy and dynamic light scattering.

Item Type: Article
Uncontrolled Keywords: casein algorithm article chemistry micelle molecular weight ultracentrifugation viscosity Algorithms Caseins Micelles
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
Related URLs:
References:

Language of Original Document: English
Correspondence Address: Morris, G.A.; NCMH Unit, School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, United Kingdom; email: scxgam@nottingham.ac.uk
Chemicals/CAS: Caseins; Micelles
References: Dickinson, E., Stainsby, G., (1982) Colloids in Food, , Applied Science Publishers: London, Chapter 8; Srinivasan, M., Singh, H., Munro, P.A., (1996) J. Agric. Food Chem., 44, p. 3807; Dewan, R.K., Bloomfield, V.A., Chudgar, A., Morr, C.V., (1973) J. Dairy Sci., 56, p. 699; McGann, T.C.A., Pyne, G.T., (1970) J. Dairy Res., 27, p. 403; Langerendorf, V., Cuvelier, G., Launay, B., Michon, A., Parker, A., De Kruif, C.G., (1999) Food Hydrocolloids, 13, p. 211; Holt, C., (1975) Biochim. Biophys. Acta, 400, p. 292; Walstra, P., (1999) Int. Dairy J., 9, p. 189; Pedersen, K.O., (1936) Biochem. J., 30, p. 948; Von Hippel, P.H., Waugh, D.F., (1955) J. Am. Chem. Soc., 77, p. 4311; Swaisgood, H.E., Brunner, J.R., (1962) J. Dairy Sci., 45, p. 1; Swaisgood, H.E., Brunner, J.R., (1963) Biochem. Biophys. Res. Commun., 12, p. 148; Swaisgood, H.E., Brunner, J.R., Lillevik, H.A., (1964) Biochemistry, 3, p. 1616; Thompson, M.P., Pepper, L., (1962) J. Dairy Sci., 45, p. 795; Pepper, L., Thompson, M.P., (1963) J. Dairy Sci., 46, p. 764; Zittle, C.A., Thompson, M.P., Custer, J.H., Cerbulis, J., (1962) J. Dairy Sci., 45, p. 807; Waugh, D.F., Creamer, L.K., Slattery, C.W., Dresden, G.W., (1970) Biochemistry, 9, p. 786; Thompson, M.P., Kallan, E.B., Greenberg, R., (1967) J. Dairy Sci., 50, p. 767; Payens, T.A.J., Van Markwijk, B.W., (1963) Biochim. Biophys. Acta, 71, p. 517; Payens, T.A.J., Brinkhuis, J.A., Van Markwijk, B.W., (1969) Biochim. Biophys. Acta, 175, p. 434; Parry R.M., Jr., Carroll, R.J., (1969) Biochim. Biophys. Acta, 194, p. 138; Buchheim, W., Schmitt, D.G., (1979) J. Dairy Res., 46, p. 277; Farrell H.M., Jr., Wickham, E.D., Dower, H.J., Pistrowski, E.G., Hoagland, P.D., Cooke, P.H., Groves, M.L., (1999) J. Protein Chem., 18, p. 637; Payens, T.A.J., (1979) J. Dairy Res., 46, p. 291; Sullivan, R.A., Fitzpatrick, M.M., Stanton, E.K., Annino, R., Kissel, G., Palermiti, F., (1955) Arch. Biochem. Biophys., 55, p. 455; Waugh, D.F., Von Hippel, P.H., (1956) J. Am. Chem. Soc., 78, p. 4576; Lin, S.H.C., Dewan, R.K., Bloomfield, V.A., Morr, C.V., (1971) Biochemistry, 10, p. 4788; Dewan, R.K., Chudgar, A., Mead, R., Bloomfield, V.A., Morr, C.V., (1974) Biochim. Biophys. Acta, 342, p. 313; Stafford, W.F., (1992) Analytical Ultracentrifugation in Biochemistry and Polymer Science, p. 359. , Harding, S. E., Rowe, A. J., Horton, J. C., Eds.; Royal Society of Chemistry: Cambridge, U.K., Chapter 20; Gralen, N., (1944), Ph.D. Dissertation, University of Uppsala, Uppsala, SwedenWales, M., Van Holde, K.E., (1954) J. Polym, Sci., 14, p. 81; Cheng, P.Y., Schachman, H.K., (1955) J. Polym. Sci., 16, p. 19; Creeth, J.M., Knight, C.G., (1965) Biochem. Biophys. Acta, 102, p. 549; Rowe, A.J., (1977) Biopolymers, 16, p. 2595; Rowe, A.J., (1992) Analytical Ultracentrifugation in Biochemistry and Polymer Science, p. 396. , Harding, S. E., Rowe, A. J., Horton, J. C., Eds.; Royal Society of Chemistry: Cambridge, U.K., Chapter 21; Ralston, G., (1993) Introduction to Analytical Ultracentrifugation, , Beckman Instruments Inc.: Fullerton: CA; Perkins, S.J., (1986) Eur. J. Biochem., 157, p. 169; Van Holde, K.E., (1985) Physical Biochemistry; 2nd Ed., , Prentice-Hall: Englewood Cliffs, NJ, Chapter 9; Huggins, M.L., (1942) J. Am. Chem. Soc., 64, p. 2716; Pavlov, G.M., (1997) Eur. Biophys. J., 25, p. 385; Harding, S.E., (1998) Prog. Biophys. Mol. Biol., 68, p. 207; Einstein, A., (1911) Ann. Phys., 34, p. 591; Svedberg, T., Pedersen, K.O., (1940) The Ultracentrifuge, , Oxford University Press: Oxford, U.K; Harding, S.E., (1999) Protein: A Comprehensive Treatise, 2, p. 271. , Chapter 7

Depositing User: Gordon Morris
Date Deposited: 13 May 2011 12:22
Last Modified: 04 Jul 2011 14:15
URI: http://eprints.hud.ac.uk/id/eprint/10436

Item control for Repository Staff only:

View Item

University of Huddersfield, Queensgate, Huddersfield, HD1 3DH Copyright and Disclaimer All rights reserved ©