UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected

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Morris, Gordon, Ebringerova, A., Harding, S. E. and Hromadkova, Z. (1999) UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected. Progress in Colloid and Polymer Science, 113. pp. 201-204. ISSN 0340-255X

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Official URL: http://dx.doi.org/10.1007/3-540-48703-4_29

DOI: 10.1007/3-540-48703-4_29

Abstract

The ultracentrifuge is a useful tool for probing the effects of chemical or other types of substitution/mutation in macromolecules, although very few studies have been performed on polysaccharides. In this study we demonstrate that the substitution of hydroxyl groups on the polysaccharide xylan by p-carboxy-benzyl (CB) bromide groups has little observed effect on molecular weight and little apparent effect on conformation (as monitored by sedimentation velocity and intrinsic viscosity). Corn-cob arabino-(4-O-methylglucurono)xylan (AGX) and its chemically modified derivative CB-AGX were found to have intrinsic viscosities of (82.8 Â± 0.4) ml/g and (77.6 Â± 0.5) ml/g, respectively. Sedimentation coefficients, s20,w0, were calculated to be (1.72 Â± 0.06) S and (1.77 Â± 0.06) S and (weight-average) molecular weights, Mw, from sedimentation equilibrium were found to be (37000 Â± 1500) g/mol and (40000 Â± 3000) g/mol. From these results we concluded that the UV tagging of arabinoxylan AGX does not significantly change the structural integrity of the xylan molecule; however, the decrease in intrinsic viscosity may indicate a very slight conformational change. Â© Springer-Verlag 1999.

Item Type:	Article
Uncontrolled Keywords:	Analytical ultracentrifugation Arabinoxylans Carboxybenzyl UV tagging
Subjects:	Q Science > QD Chemistry
Schools:	School of Applied Sciences
Related URLs:	Author
References:	Language of Original Document: English Correspondence Address: Morris, G.A.; NCMH Unit, School of Biological Sciences, University of Nottingham, Leicestershire LE12 5RD, United Kingdom; email: scxgam@szn1.agric.nottingham.ac.uk References: De Gruyter, G., (1984) Wood Chemistry, Ultrastructure, Reaction, , Springer, Berlin Heidelberg, New York; Tombs, M.P., Harding, S.E., (1998) An Introduction to Polysaccharide Biotechnology, , Taylor and Francis, London; Stephen, A.M., (1995) Food Polysaccharides and Their Applications, , Dekker, New York; Ebringerova, A., Novotna, M., Kacurakova, M., Machova, E., (1996) J App Polym Sci, 6, p. 1043; Ebringerova, A., Simkovic, I., Hromadkova, H., Toman, R., (1988) Chem Abs, 109, pp. 8277k. , Czech Patent CS 244591; Kohn, R., Hromadkova, H., Ebringerova, A., Toman, R., (1986) Collect Czech Chem Commun, 51, p. 2243; Cassassa, E.F., Eisenberg, H., (1964) Adv Protein Chem, 19, p. 287; Green, A.A., (1933) J Am Chem Soc, 55, p. 2331; Ralston, G., (1993) Introduction to Analytical Ultracentrifugation, , Beckman Instruments, Fullerton, USA; Dhami, R., Harding, S.E., Elizabeth, N.J., Ebringerova, A., (1995) Carbohydr Polym, 28, p. 113; Stafford, W.F., (1992) Analytical Ultracentrifugation in Biochemistry and Polymer Science, pp. 359-393. , Harding SE, Rowe AJ, Horton JC (eds) Royal Society of Chemistry, Cambridge; Pavlov, G.M., (1997) Eur Biophys J, 25, p. 385; Gralen, N., (1944), PhD dissertation. University of Uppsala, SwedenCÃ¶lfen, H., Harding, S.E., (1997) Eur Biophys J, 25, p. 333; Errington, N.C., Harding, S.E., Rowe, A.J., (1992) Carbohydr Polym, 17, p. 151; Errington, N.C., Harding, S.E., Illum, L., Schacht, E.H., (1992) Carbohydr Polym, 18, p. 289
Depositing User:	Gordon Morris
Date Deposited:	13 May 2011 12:26
Last Modified:	04 Jul 2011 14:39
URI:	http://eprints.hud.ac.uk/id/eprint/10435

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