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UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected

Morris, Gordon, Ebringerova, A., Harding, S. E. and Hromadkova, Z. (1999) UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected. Progress in Colloid and Polymer Science, 113. pp. 201-204. ISSN 0340-255X

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Abstract

The ultracentrifuge is a useful tool for probing the effects of chemical or other types of substitution/mutation in macromolecules, although very few studies have been performed on polysaccharides. In this study we demonstrate that the substitution of hydroxyl groups on the polysaccharide xylan by p-carboxy-benzyl (CB) bromide groups has little observed effect on molecular weight and little apparent effect on conformation (as monitored by sedimentation velocity and intrinsic viscosity). Corn-cob arabino-(4-O-methylglucurono)xylan (AGX) and its chemically modified derivative CB-AGX were found to have intrinsic viscosities of (82.8 ± 0.4) ml/g and (77.6 ± 0.5) ml/g, respectively. Sedimentation coefficients, s20,w0, were calculated to be (1.72 ± 0.06) S and (1.77 ± 0.06) S and (weight-average) molecular weights, Mw, from sedimentation equilibrium were found to be (37000 ± 1500) g/mol and (40000 ± 3000) g/mol. From these results we concluded that the UV tagging of arabinoxylan AGX does not significantly change the structural integrity of the xylan molecule; however, the decrease in intrinsic viscosity may indicate a very slight conformational change. © Springer-Verlag 1999.

Item Type: Article
Uncontrolled Keywords: Analytical ultracentrifugation Arabinoxylans Carboxybenzyl UV tagging
Subjects: Q Science > QD Chemistry
Schools: School of Applied Sciences
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Language of Original Document: English
Correspondence Address: Morris, G.A.; NCMH Unit, School of Biological Sciences, University of Nottingham, Leicestershire LE12 5RD, United Kingdom; email: scxgam@szn1.agric.nottingham.ac.uk
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Depositing User: Gordon Morris
Date Deposited: 13 May 2011 12:26
Last Modified: 04 Jul 2011 14:39
URI: http://eprints.hud.ac.uk/id/eprint/10435

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