Morris, Gordon, Ebringerova, A., Harding, S. E. and Hromadkova, Z. (1999) UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected. Progress in Colloid and Polymer Science, 113. pp. 201-204. ISSN 0340-255XMetadata only available from this repository.
The ultracentrifuge is a useful tool for probing the effects of chemical or other types of substitution/mutation in macromolecules, although very few studies have been performed on polysaccharides. In this study we demonstrate that the substitution of hydroxyl groups on the polysaccharide xylan by p-carboxy-benzyl (CB) bromide groups has little observed effect on molecular weight and little apparent effect on conformation (as monitored by sedimentation velocity and intrinsic viscosity). Corn-cob arabino-(4-O-methylglucurono)xylan (AGX) and its chemically modified derivative CB-AGX were found to have intrinsic viscosities of (82.8 Â± 0.4) ml/g and (77.6 Â± 0.5) ml/g, respectively. Sedimentation coefficients, s20,w0, were calculated to be (1.72 Â± 0.06) S and (1.77 Â± 0.06) S and (weight-average) molecular weights, Mw, from sedimentation equilibrium were found to be (37000 Â± 1500) g/mol and (40000 Â± 3000) g/mol. From these results we concluded that the UV tagging of arabinoxylan AGX does not significantly change the structural integrity of the xylan molecule; however, the decrease in intrinsic viscosity may indicate a very slight conformational change. Â© Springer-Verlag 1999.
|Uncontrolled Keywords:||Analytical ultracentrifugation Arabinoxylans Carboxybenzyl UV tagging|
|Subjects:||Q Science > QD Chemistry|
|Schools:||School of Applied Sciences|
Language of Original Document: English
|Depositing User:||Gordon Morris|
|Date Deposited:||13 May 2011 12:26|
|Last Modified:||04 Jul 2011 14:39|
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