Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide

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Morris, Gordon, Butler, S. N. G., Foster, T. J., Jumel, K. and Harding, S. E. (1999) Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide. Progress in Colloid and Polymer Science, 113. pp. 205-208. ISSN 0340-255X

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Official URL: http://dx.doi.org/10.1007/3-540-48703-4_30

DOI: 10.1007/3-540-48703-4_30

Abstract

Relatively little has been published on the ultracentrifuge behaviour of macromolecular solutions at elevated temperature (> 40Â°C). In this study we look at the sedimentation velocity behaviour of one particular food grade polyuronide - pectin - from 20Â°C to 60Â°C in a specially adapted Model E ultracentrifuge. Reduced specific viscosity measurements were also determined over the same temperature range. A small decrease in the reduced viscosity, together with a similar increase in s20,w, suggests that the pectin chain is more flexible at elevated temperatures, but that the overall molecular integrity remains intact. Â© Springer-Verlag 1999.

Item Type:	Article
Uncontrolled Keywords:	Beckman Model E adaptation Size exclusion chromatography-multiangle laser light scattering Viscometry
Subjects:	Q Science > QD Chemistry
Schools:	School of Applied Sciences
Related URLs:	Author
References:	Language of Original Document: English Correspondence Address: Morris, G.A.; NCMH Unit, School of Biological Sciences, University of Nottingham, Sutton Bonington, Leics. LE12 5RD, United Kingdom; email: scxgam@szn1.agric.nottingham.ac.uk References: Tombs, M.P., Harding, S.E., (1998) An Introduction to Polysaccharide Biotechnology, , Taylor and Francis, London; Green, A.A., (1933) J Am Chem Soc, 25, p. 2331; Pavlov, G.M., (1997) Eur Biophys J, 25, p. 385; (1974) Beckman Spinco Service Manual, , Beckman Instruments, Palo Alto, Calif; Van Holde, K.E., (1985) Physical Bochem-istry, 2nd Edn., , Prentice-Hall, Englewood Cliffs, NJ; Jumel, K., Browne, P., Kennedy, J.F., (1992) Laser Light Scattering in Biochemistry, , Harding SE, Sattelle DB, Bloomfeld VA (eds) chap 2. Royal Society of Chemistry, Cambridge; Jumel, K., (1994), Ph D Thesis, University of NottinghamWyatt, P.J., (1992) Laser Light Scattering in Biochemistry, , Harding SE, Sattelle DB, Bloomfeld VA (eds) chap 3. Royal Society of Chemistry, Cambridge; Harding, S.E., Berth, G., Ball, A., Mitchell, J.R., Garcia De La Torre, J., (1991) Carbohydr Polym, 16, p. 1
Depositing User:	Gordon Morris
Date Deposited:	19 May 2011 13:00
Last Modified:	04 Jul 2011 14:37
URI:	http://eprints.hud.ac.uk/id/eprint/10434

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